Luminex® Assays High-throughput Multiplex Bead Based Assays
ثبت نشده
چکیده
Panomics has an aggressive program for the development of a broad range of assays for the Luminex platform and similar instruments based on the xMAP technology. Currently we have hundreds of RNA targets available in 3–30 plex assays using our using our QuantiGene Plex Reagent Systems, with new targets being added weekly. Our Procarta® Human, Mouse and Rat Assays can quantitatively measure cytokines and chemokines from a variety of sample sources, including serum and plasma. We’ve recently expanded our family of assays designed to measure protein expression and monitor protein modifications/activation states in diverse matrices.
منابع مشابه
Evaluation of the Luminex xTAG Respiratory Viral Panel FAST v2 assay for detection of multiple respiratory viral pathogens in nasal and throat swabs in Vietnam
BACKGROUND Acute respiratory infections (ARI) are among the leading causes of hospitalization in children ≤5 years old. Rapid diagnostics of viral pathogens is essential to avoid unnecessary antibiotic treatment, thereby slowing down antibiotic-resistance. We evaluated the diagnostic performance of the Luminex xTAG Respiratory Viral Panel FAST v2 against viral specific PCR as reference assays f...
متن کاملDevelopment of a bead-based multiplex assay for simultaneous quantification of cytokines in horses.
The detection and quantification of equine cytokines has been hampered by the lack of antibodies for many years. With the development of antibody pairs for equine cytokines during the past years, the quantification of these essential regulators of the immune response became possible. After being successfully tested by enzyme-linked immunosorbent assays (ELISA), three of these anti-cytokine reag...
متن کاملCombined serology and antibiotic residue detection in a Luminex assay
Serum of slaughter pigs is being used for routine testing of antibodies against pathogens and can also be used for detection of antibiotic residues. Since the assay formats differ (direct detection of antibodies vs. inhibition assays for residues), these tests are performed in parallel, not in a multiplex set-up. For this study, a protocol for testing both antibodies and residues in one sample ...
متن کاملAn Evaluation of Commercial Fluorescent Bead-Based Luminex Cytokine Assays
The recent introduction of fluorescent bead-based technology, allowing the measurement of multiples analytes in a single 25-50 microl sample has revolutionized the study of cytokine responses. However, such multiplex approaches may compromise the ability of these assays to accurately measure actual cytokine levels. This study evaluates the performance of three commercially available multiplex c...
متن کاملOverview of Luminex Assay Performance
Luminex Assay Design and Methods. Luminex assays use antibody-conjugated bead sets to detect analytes in a multiplexed sandwich immunoassay format. Each bead in the set is identified by a unique content of two addressing dyes, with a third dye used to read out binding of the analyte via a biotin-conjugated antibody and streptavidin-conjugated second step detector. Data is acquired on a dedicate...
متن کامل